Search results for "Granzyme A"

showing 5 items of 5 documents

Granzyme A as a potential biomarker of Mycobacterium tuberculosis infection and disease

2015

Cytotoxic molecules such as granulysin, perforin and granzymes produced by cytolytic T cells directly contribute to immune defense against tuberculosis (TB). In search for novel TB biomarkers, we have evaluated the levels of granzyme A in plasma obtained from QuantiFERON-TB Gold In tube (QFT-IT) assays from patients with active TB disease and subjects with latent TB infection (LTBI).Granzyme A serum levels in TB patients were significantly lower than values found in LTBI subjects even after subtraction of the unstimulated levels from the antigen-stimulated responses. The receiver operator characteristics (ROC) curve analysis comparing TB patients and LTBI groups, showed that at a cut-off va…

AdultMaleTuberculosisTuberculosiImmunologyDisease; Granzyme A; IFN-γ; IGRA tests; Infection; Tuberculosis; Adult; Antigens Bacterial; Biomarkers; Case-Control Studies; Female; Granzymes; Humans; Interferon-gamma; Latent Tuberculosis; Male; Middle Aged; Mycobacterium tuberculosis; ROC Curve; Tuberculosis; Young Adult; Immunology and Allergy; ImmunologyMycobacterium tuberculosiIFN-γGranzymesMycobacterium tuberculosisIGRA testInterferon-gammaYoung AdultLatent TuberculosismedicineHumansTuberculosisImmunology and AllergyDiseaseGranulysinGranzymeAntigens BacterialLatent TuberculosibiologyLatent tuberculosisGranzyme AMycobacterium tuberculosisBiomarkerMiddle Agedbacterial infections and mycosesmedicine.diseasebiology.organism_classificationROC CurvePerforinGranzymeCase-Control StudiesImmunologybiology.proteinGranzyme ABiomarker (medicine)FemaleInfectionCase-Control StudieBiomarkersHuman
researchProduct

IL-10 Controls Ultraviolet-Induced Carcinogenesis in Mice

2007

Abstract UV radiation-induced immunosuppression contributes significantly to the development of UV-induced skin cancer by inhibiting protective immune responses. IL-10 has been shown to be a key mediator of UV-induced immunosuppression. To investigate the role of IL-10 during photocarcinogenesis, groups of IL-10+/+, IL-10+/−, and IL-10−/− mice were chronically irradiated with UV. IL-10+/+ and IL-10+/− mice developed skin cancer to similar extents, whereas IL-10−/− mice were protected against the induction of skin malignancies by UV. Because UV is able to induce regulatory T cells, which play a role in the suppression of protective immunity, UV-induced regulatory T cell function was analyzed…

Neoplasms Radiation-InducedSkin NeoplasmsUltraviolet RaysRegulatory T cellImmunologyMice NudeBiologymedicine.disease_causeT-Lymphocytes RegulatoryMiceImmune systemImmunityImmune TolerancemedicineAnimalsImmunology and AllergyIL-2 receptorMice KnockoutMolecular biologyInterleukin-10Mice Inbred C57BLInterleukin 10medicine.anatomical_structureImmunologyGranzyme ACytokinesCarcinogenesisCD8The Journal of Immunology
researchProduct

Phenotype and functional changes of Vγ9/Vδ2 T lymphocytes in Behçet's disease and the effect of infliximab on Vγ9/Vδ2 T cell expansion, activation an…

2010

Introduction: Infliximab is a chimeric monoclonal antibody against tumor necrosis factor alpha (TNF-α) that has been introduced recently for Behcet's disease (BD) patients who were resistant to standard treatment. The aim of this study was to analyse the functional changes of Vγ9/Vδ2 T lymphocytes in both active and inactive disease and the effect of infliximab on Vγ9/Vδ2 T cell expansion, activation and cytotoxicity. Methods: We investigated 1) cell expansion, 2) expression of TNFRII receptor, 3) perforin and gamma interferon (IFN) content, 4) release of granzyme A (GrA) and 5) phenotype changes, in vitro and in vivo, in Vγ9/Vδ2 T lymphocytes by means of fluorescence-activated cell sorter …

AdultAdolescentCell SurvivalT cellLymphocyteT-LymphocytesImmunologyGranzymesInterferon-gammaYoung AdultRheumatologyAntigenmedicineImmunology and AllergyHumansReceptors Tumor Necrosis Factor Type IIInterferon gammaCells CulturedAgedCell ProliferationbiologyPerforinBehcet SyndromeAntibodies MonoclonalReceptors Antigen T-Cell gamma-deltaMiddle AgedInfliximabmedicine.anatomical_structurePhenotypeGranzymePerforinAntirheumatic AgentsCase-Control StudiesImmunologybiology.proteinGranzyme ATumor necrosis factor alphaFemalemedicine.drugResearch ArticleArthritis Research & Therapy
researchProduct

Granzyme A is an additional marker for tuberculosis (TB) to discriminate between patients with active disease and subjects with latent infection.

2013

TuberculosisTuberculosis granzyme A ELISA LTBI Cytotoxic molecules TB patientsbusiness.industryImmunologyImmunologyActive diseaseGranzyme AmedicineImmunology and Allergymedicine.diseasebusinessVirologyFrontiers in Immunology
researchProduct

Reduction of plasma granzyme A correlates with severity of sepsis in burn patients.

2009

The risk of mortality is high in burn patients and correlates with age, burn area extent, and sepsis. Immunosuppression has been reported to occur after severe burn. Cytotoxic cells possess specialized granules containing perforin and a group of serine proteases (granzymes). Granzyme A is a serine protease constitutively expressed by gammadelta and NK cells, in agreement with their functional cytolytic potential. In vitro studies have shown that GrA may be released extracellularly during cytotoxic cell degranulation, indicating the activation of cytotoxic cells. The aim of our study was to determine plasma GrA activity in burned patients and to verify if decreased GrA levels were associated…

AdultMaleProteasesCritical Care and Intensive Care MedicineGranzymesNatural killer cellSepsisSepsisparasitic diseasesmedicineCytotoxic T cellHumansAgedRetrospective Studiesbiologybusiness.industryDegranulationGeneral MedicineMiddle Agedmedicine.diseasePrognosisAnti-Bacterial Agentsmedicine.anatomical_structurePerforinGranzymeImmunologyEmergency Medicinebiology.proteinGranzyme ASurgeryFemalebusinessBurnsBiomarkersBurns : journal of the International Society for Burn Injuries
researchProduct